No filter change with dual monochromator optics. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. ValitaTiter Plus 96-well (1 plate) VAL004. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. Molecular Devices' microplate readers. 63. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. book Page 9 Wednesday, July 7, 2010 2:09 PM. Technical specifications. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Dynamic range > 9 orders of magnitude. M3-M5e_manual. 2 Pages. Photometric and kinetic analysis mode. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. ValitaTiter Plus 96-well (1 plate) VAL004. Other technologies such as imaging. 100 fmol 1536-well (8 µL) 0. Eight filter modes enable applications such as protein quantification, cell viability and ELISA. ’s GloMax®-96, for instance, is a dedicated luminometer, whereas Bio-Rad Laboratories’ iMark and xMark strictly measure absorbance. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. When reading at wavelengths. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. 03 pM 384-well: 0. 96-well solid bottom, clear polystyrene microplates (Greiner cat. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a black, clearbottom microplate. This page contains the. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. Molecular Devices has provided scientists with . Four wash/rinse bottles are included and configurable for both 96- and 384. ZERO BIAS. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Molecular Devices is a leader in comprehensive compliance solutions with microplate detection systems. A microplate reader as . First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. . DNA concentration (ng/mL) Average RFU. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Incubate the plate at 37 °C for approximately one hour. Endpoint, kinetic, spectrum and area-well scanning Click here. Cross-talk < 0. 125 pM. These models can no longer be repaired. Reading a Microplate . 2. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). Plate readers, also known as microplate readers or microplate photometers,. 384 Awareness Technology ChroMate BioTek Synergy Mx. 03 pM 384-well: 0. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. A multitude of modalities. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Molecular Devices provides. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. , Sigma cat. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. #SpectraMax L Config) Methods. Taking into account the price differences between each seller and each country. Expt#3 . We would like to show you a description here but the site won’t allow us. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. *Simple analysis and optimization. Features. 96-well microplate compatible Well read area range: D4 to F11 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-VolumeLearn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. The contents of the SpectraDrop Microplate kit depend on kit type. 1536-well: 0. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 3. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. Model:. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. SpectraMax 250 User. 2 nm Wavelength Bandpass 2. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. We would like to show you a description here but the site won’t allow us. Average StDev (mP) 1. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Use the provided 3. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. Integrated stacker option. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. 96-well: 0. Next generation SpectraMax® absorbance microplate readers. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. This reader's dual monochromators for. 76 L x 85. Highly selective for dsDNA over ssDNA or RNA. Materials. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. With various on-board features and. Less background noise gives a better limit of detection and so a relative increase in usable signal. Multi-Mode Microplate Reader for . Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 1 OD at 230 nm. These instruments are all elegant and simple in their form and function. (Molecular Devices cat. Paradigm: Delta mP. 1-color 96-well . Keywords: fluorescence polarization, trf, fret, igg. No accessories are needed and SoftMax® Pro Software. Please click on the links below to view the Microplate Reader Comparison Tables. Standard SBS 96 and 384 well plates (128. , Sigma cat. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. , 384 wells < 45 sec. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. It also integrates with the powerful SoftMax Pro Software for. Both readers were heated to 37ºC prior to setting up the assay. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Documentation:1. The iMark. The Absorbance 96 is a uniquely designed microplate reader for assays in 96-well format. softmaxt pro microplate data analysis, molecular devices’ stakmax microplate stacker, monochromator-based fluorimeter, wavelength optimization, fda 21 cfr part 11. 96- or 384-well microplate compatibility. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 125 pM * Specifications listed are for europium 96-well: 0. Temperature control. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Setup Guide. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. We would like to show you a description here but the site won’t allow us. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. 100 μL/well Steady-Luc working solution was added to the wells. Description. Microplate Formats: 6, 12, 24, 48, 96, 384. NanoRacks added it to their line of commercial . $489. Determine DNA concentrations precisely and . 5 mm and 15. A 1:10 serial dilution of ATP was prepared in medium. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Published Dec 16, 2020 | Updated Dec 16, 2020. Growth medium was then replaced with fatty acid uptake solution. 05. The spectrophotometer has a wide wavelength range 190. SOFTmax PRO allows you to select excitation and emission values from the literature and simply input their values for the monochromator settings. Monochromator, tunable in 1-nm increments. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Making it possible to perform kinetic analysis of solid. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. With various on-board features and capabilities, the ThermoMax is. SOP Title: Use and. 0 GB Brand Hewlett-Packard Hard driveThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 92: 88. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 30 °C. Molecular Devices VersaMax Microplate Reader Specifications. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. 384-well: 0. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. absorbance, fluorescence, and luminescence with the added functionality of. 7°C and the bottom to 36. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. Built in near field communication (NFC). Microscopy. As a result, assay flexibility is consequence-free. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. Supplier: Molecular Devices. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. Suggested plates are black walled. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). 2. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. Use standard plate unless you see your type listed. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. #N207) LAL Reagent Water (Lonza cat. com. The concentration of total free thiols in the protein. (Figure 1). With theThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. accurately over a wide dynamic range. 48 W x 12. 3. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. #ABS PLUS) Methods. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. g. Microplate Reader (Molecular Devices cat. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Microplate readers use a vertical light path so the distance of the. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Microplate readers use a vertical light path so the distance of the. 2600A October 2023. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. The drawing shows a standard microplate of 15 mm height covered with a lid. Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Danaher Inc 96 well microplate reader 96 Well Microplate Reader, supplied by Danaher Inc, used in various techniques. absorbance, fluorescence, and luminescence with the added functionality of. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. An industry-renowned instrument for monitoring GPCRs and ion channels. i3x. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. After 5, 7, 11, and 13 days. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Microplate Readers. You can add your own protocols. flat meniscus), but is approx 15 mL. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. automation for any lab. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. GxP regulated industry assessments of microplate readers. i3x. This unit offers a wavelength range of 340 nm to 850 nm and a bandwidth of 5 nm. Software Validation. No additional manuals are available. tecHnical SPecificationS Performance Specifications Measurement range: 400–750 nm 0–4. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. ” Promega Corp. 003 fmol 0. About;. 96-well microplate at 200 µL/well. Optical System: High energy xenon flash lamp. 03 pM. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Equipped with scanning, shaking and kinetics modules. added to 96-well at 37 °C for 2 h, then the absorbance was measured at 450 nm by a. Molecular Devices i3x. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. 2 OD at 200 nm; this decreased to 0. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. High. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. High tolerance for contaminants. 0 OD at. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. 03 pM. g. of 0. Fluorescence Microplate Readers. style. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. 00. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. Temperature Control: ambient from +4 to 45C. g. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. Molecular Devices gmbH Tel. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Growth medium was then replaced with fatty acid uptake solution. In simplest terms, a plate reader measures and. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. Materials. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 747. An example of dual-luciferase assay results. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. At 5 seconds to read a 96-well endpoint assay, the EMax Microplate Reader has both the speed and performance to carry a heavy workload. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraDrop High-Throughput Screening (HTS) Kit. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 69: 85. 3 OD at 200 nm. 2. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Supplementary Information. #12-639) White 96- and 384-well microplates (Greiner cat. Formats available are strip washers, full plate washers and combination washer-dispensers. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. 54: 1. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. The microplate reader then generates data following a preconfigured protocol that performs the necessary calculations on the data, delivering consistent results. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. 2 OD at 200 nm; this decreased to 0. For biochemical. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. tools to expand the boundaries of their research. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Wavelength Range 200 to 999 nm. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL.